{"schema":"human-passive-validation-v1","dataset_id":"dandi-000293-18201004","specimen":{"id":"18201004","species":"Homo sapiens","region":"Left-ATL · L5；作者元数据，不代表分子细胞类型","condition":"癫痫手术切片；Synaptic Blockers；NMDG切片液；未校正bridge balance","source_url":"https://dandiarchive.org/dandiset/000293/0.220708.1652","identity_status":"论文人体子集、NWB和作者CSV一致；NWB subject1802-18201004为供者与记录组合标识；没有形态匹配"},"protocol":{"version":"dandi-passive-rc-zoh-1","status":"frozen before response waveform arrays","created_at_unix":1789558619.178911,"dataset_version":"000293/0.220708.1652","asset_id":"44eb6883-68b8-46ce-a15c-3a32b4fe545b","nwb_file":"sub-1802-18201004_icephys.nwb","nwb_sha256":"e8d2c480b25b794bc39d19bb5c2e72d5e28d9d597100f624e30e5271327616fd","expected_nwb_bytes":715794,"recording_id":"18201004","subject_id":"1802-18201004","metadata_commit":"4d96fd5fc31438c5110c0521b53012f1e7d6c6e6","metadata_url":"https://raw.githubusercontent.com/derekhoward/nwb_conversion/4d96fd5fc31438c5110c0521b53012f1e7d6c6e6/data/processed/meta/metadata.csv","metadata_sha256":"84ea74e9c46d847c4f3f30ba1c5398f69c603f14e86f5add96951f054d843a1b","training_sweeps":[0,2],"test_sweeps":[1],"split_rule":"All three negative-current sweeps; outer amplitudes train, middle amplitude test, chosen from stimulus only. Positive sweeps are not analyzed.","preexisting_information":"Public RMP/QC summaries and source comments were visible; not claimed to be completely blind external validation.","model":"single-compartment passive RC, actual holding-subtracted current samples under zero-order hold; no spikes, synapses or morphology coupling","equation":"V[k+1]=E+(V[k]-E)*exp(-dt/tau)+(R_MOhm/1000)*(1-exp(-dt/tau))*I_pA[k]","parameter_constraints":{"tau_ms":[0.5,200],"tau_log_grid_points":401,"resistance_MOhm":[5,2000],"equilibrium_mV":[-120,-20]},"inputs":{"0":{"onset_index":372,"offset_index_exclusive":10372,"prediction_start_index":100,"prediction_end_index_exclusive":12372,"initial_past_indices":[0,100],"holding_pA":-0.081787109375,"plateau_relative_pA":-49.84497070312499,"rate_Hz":10000.0,"stimulus_current_sha256_float64_pA":"c2d2c18dd71733ba7c73fccb47f11d5c01b1090447e69658248ca85931925f5f"},"1":{"onset_index":372,"offset_index_exclusive":10372,"prediction_start_index":100,"prediction_end_index_exclusive":12372,"initial_past_indices":[0,100],"holding_pA":-0.155029296875,"plateau_relative_pA":-29.874267578125,"rate_Hz":10000.0,"stimulus_current_sha256_float64_pA":"0ad408e2cfdf2aeb00eecef73caf82bdf5f0105e0b591f4adb561c76b6e46a63"},"2":{"onset_index":372,"offset_index_exclusive":10372,"prediction_start_index":100,"prediction_end_index_exclusive":12372,"initial_past_indices":[0,100],"holding_pA":-0.159912109375,"plateau_relative_pA":-9.849853515625,"rate_Hz":10000.0,"stimulus_current_sha256_float64_pA":"c1f8a1b13cdc49b6927a22590a5fedaf01127efe9027f0594ec44a9bd7dadfe5"}},"score_windows_ms":{"0":[[29.2,1027.2],[1029.2,1227.2]],"1":[[29.2,1027.2],[1029.2,1227.2]],"2":[[29.2,1027.2],[1029.2,1227.2]]},"initial_state":"Mean V over original samples [0,100), strictly before prediction from sample100 (10ms); same causal rule in train/test.","observation_map":"NWB2 data*conversion+offset, then volts->mV and amperes->pA; no second gain/offset correction. Sampled voltage at original time nodes. No smoothing or resampling.","controls":{"frozen_engine_passive":{"equilibrium_mV":-70,"resistance_MOhm":80,"tau_ms":20,"capacitance_pF":250},"constant_past_baseline":"V(t)=same V0; no fitted parameters"},"metrics":["RMSE","MAE","bias","maximum absolute error","observed/predicted Delta","test error improvement against both controls"],"delta_windows":"mean last20ms before pulse offset minus mean past [0,10)ms, mV; distinct from observed-predicted residual","uncertainty":"tau training-loss profile plus separately fitting each train amplitude; descriptive ranges, not population CI or separation of noise/model error","failures":"Nonfinite/mismatched units/timeline/hash abort. Training scored voltage >=-20mV aborts passive fit. Test depolarization >=-20mV is flagged unsuitable and scored without adaptive masking/refit.","limitations":["No bridge correction; effective parameters include possible electrode effects","Only one cell/one held-out amplitude; no donor-population inference","No unique identification of ion channels, noise, or unknown dynamics","NWB data contain prior author gain/offset calibration; original ABF not independently re-converted"],"limits":{"max_samples_per_sweep":25000,"max_selected_sweeps":3,"max_display_points_per_sweep":3000,"compute_threads":1,"gpu":false,"estimated_worker_peak_mib":256,"estimated_project_with_browser_allowance_mib":768,"planned_job_wall_seconds":90},"model_type":"single-compartment passive RC, actual holding-subtracted current samples under zero-order hold; no spikes, synapses or morphology coupling","fit_window":{"0":[[29.2,1027.2],[1029.2,1227.2]],"1":[[29.2,1027.2],[1029.2,1227.2]],"2":[[29.2,1027.2],[1029.2,1227.2]]}},"parameters":{"equilibrium_mV":-55.36819019478834,"resistance_MOhm":459.64038939987324,"tau_ms":23.136006561666875,"capacitance_pF":50.33501645030426,"source":"训练记录0与2的实际电流驱动被动RC拟合；401点tau预定网格和有界E/R最小二乘","uncertainty":{"meaning":"tau training-loss profile plus separately fitting each train amplitude; descriptive ranges, not population CI or separation of noise/model error","per_training_amplitude_ranges":{"equilibrium_mV":[-55.22480847083349,-54.40664836932324],"resistance_MOhm":[478.7868112181393,495.2362139101458],"tau_ms":[24.199358237331577,28.964474161335296],"capacitance_pF":[50.54307610471364,58.48617962052049]},"individual_fits":[{"sweep_id":0,"params":{"equilibrium_mV":-54.40664836932324,"resistance_MOhm":478.7868112181393,"tau_ms":24.199358237331577,"capacitance_pF":50.54307610471364}},{"sweep_id":2,"params":{"equilibrium_mV":-55.22480847083349,"resistance_MOhm":495.2362139101458,"tau_ms":28.964474161335296,"capacitance_pF":58.48617962052049}}]}},"validation":{"status":"已完成有限单细胞留出评估；误差见记录","notes":["训练0/2，测试1；测试评分窗口电压只在全部预测存盘后读取。","已看过公开RMP/QC摘要，不称完全盲法外部验证。","软件解析与保存核对不证明模型完整复刻人体细胞。","同窗报告冻结工程被动参数与常值过去基线对照；未据测试分数调参。","留出记录1：拟合模型 RMSE 1.4783 mV；固定工程参数对照 6.6383 mV；常值过去基线 14.2870 mV。相同输入、初值与评分窗口。"]},"sweeps":[{"id":0,"split":"train","amplitude_pA":-49.84497070312499,"dt_ms":0.1,"original_analysis_samples":12272,"time_ms":[0.0,0.4,0.8,1.2000000000000002,1.6,2.0,2.4000000000000004,2.8000000000000003,3.2,3.6,4.0,4.5,4.9,5.300000000000001,5.7,6.1000000000000005,6.5,6.9,7.300000000000001,7.7,8.1,8.5,9.0,9.4,9.8,10.200000000000001,10.600000000000001,11.0,11.4,11.8,12.200000000000001,12.600000000000001,13.0,13.5,13.9,14.3,14.700000000000001,15.100000000000001,15.5,15.9,16.3,16.7,17.1,17.5,18.0,18.400000000000002,18.8,19.200000000000003,19.6,20.0,20.400000000000002,20.8,21.200000000000003,21.6,22.0,22.5,22.900000000000002,23.3,23.700000000000003,24.1,24.5,24.900000000000002,25.3,25.700000000000003,26.1,26.5,27.0,27.400000000000002,27.8,28.200000000000003,28.6,29.0,29.400000000000002,29.8,30.200000000000003,30.6,31.0,31.5,31.900000000000002,32.300000000000004,32.7,33.1,33.5,33.9,34.300000000000004,34.7,35.1,35.5,36.0,36.4,36.800000000000004,37.2,37.6,38.0,38.400000000000006,38.800000000000004,39.2,39.6,40.0,40.5,40.900000000000006,41.300000000000004,41.7,42.1,42.5,42.900000000000006,43.300000000000004,43.7,44.1,44.5,45.0,45.400000000000006,45.800000000000004,46.2,46.6,47.0,47.400000000000006,47.800000000000004,48.2,48.6,49.1,49.5,49.900000000000006,50.300000000000004,50.7,51.1,51.5,51.900000000000006,52.300000000000004,52.7,53.1,53.6,54.0,54.400000000000006,54.800000000000004,55.2,55.6,56.0,56.400000000000006,56.800000000000004,57.2,57.6,58.1,58.5,58.900000000000006,59.300000000000004,59.7,60.1,60.5,60.900000000000006,61.300000000000004,61.7,62.1,62.6,63.0,63.400000000000006,63.800000000000004,64.2,64.60000000000001,65.0,65.4,65.8,66.2,66.60000000000001,67.10000000000001,67.5,67.9,68.3,68.7,69.10000000000001,69.5,69.9,70.3,70.7,71.10000000000001,71.60000000000001,72.0,72.4,72.8,73.2,73.60000000000001,74.0,74.4,74.8,75.2,75.60000000000001,76.10000000000001,76.5,76.9,77.30000000000001,77.7,78.10000000000001,78.5,78.9,79.30000000000001,79.7,80.10000000000001,80.60000000000001,81.0,81.4,81.80000000000001,82.2,82.60000000000001,83.0,83.4,83.80000000000001,84.2,84.60000000000001,85.10000000000001,85.5,85.9,86.30000000000001,86.7,87.10000000000001,87.5,87.9,88.30000000000001,88.7,89.10000000000001,89.60000000000001,90.0,90.4,90.80000000000001,91.2,91.60000000000001,92.0,92.4,92.80000000000001,93.2,93.60000000000001,94.10000000000001,94.5,94.9,95.30000000000001,95.7,96.10000000000001,96.5,96.9,97.30000000000001,97.7,98.2,98.60000000000001,99.0,99.4,99.80000000000001,100.2,100.60000000000001,101.0,101.4,101.80000000000001,102.2,102.7,103.10000000000001,103.5,103.9,104.30000000000001,104.7,105.10000000000001,105.5,105.9,106.30000000000001,106.7,107.2,107.60000000000001,108.0,108.4,108.80000000000001,109.2,109.60000000000001,110.0,110.4,110.80000000000001,111.2,111.7,112.10000000000001,112.5,112.9,113.30000000000001,113.7,114.10000000000001,114.5,114.9,115.30000000000001,115.7,116.2,116.60000000000001,117.0,117.4,117.80000000000001,118.2,118.60000000000001,119.0,119.4,119.80000000000001,120.2,120.7,121.10000000000001,121.5,121.9,122.30000000000001,122.7,123.10000000000001,123.5,123.9,124.30000000000001,124.7,125.2,125.60000000000001,126.0,126.4,126.80000000000001,127.2,127.60000000000001,128.0,128.4,128.8,129.20000000000002,129.70000000000002,130.1,130.5,130.9,131.3,131.70000000000002,132.1,132.5,132.9,133.3,133.70000000000002,134.20000000000002,134.6,135.0,135.4,135.8,136.20000000000002,136.6,137.0,137.4,137.8,138.20000000000002,138.70000000000002,139.1,139.5,139.9,140.3,140.70000000000002,141.1,141.5,141.9,142.3,142.8,143.20000000000002,143.6,144.0,144.4,144.8,145.20000000000002,145.6,146.0,146.4,146.8,147.3,147.70000000000002,148.1,148.5,148.9,149.3,149.70000000000002,150.1,150.5,150.9,151.3,151.8,152.20000000000002,152.6,153.0,153.4,153.8,154.20000000000002,154.60000000000002,155.0,155.4,155.8,156.3,156.70000000000002,157.10000000000002,157.5,157.9,158.3,158.70000000000002,159.10000000000002,159.5,159.9,160.3,160.8,161.20000000000002,161.60000000000002,162.0,162.4,162.8,163.20000000000002,163.60000000000002,164.0,164.4,164.8,165.3,165.70000000000002,166.10000000000002,166.5,166.9,167.3,167.70000000000002,168.10000000000002,168.5,168.9,169.3,169.8,170.20000000000002,170.60000000000002,171.0,171.4,171.8,172.20000000000002,172.60000000000002,173.0,173.4,173.8,174.3,174.70000000000002,175.10000000000002,175.5,175.9,176.3,176.70000000000002,177.10000000000002,177.5,177.9,178.3,178.8,179.20000000000002,179.60000000000002,180.0,180.4,180.8,181.20000000000002,181.60000000000002,182.0,182.4,182.8,183.3,183.70000000000002,184.10000000000002,184.5,184.9,185.3,185.70000000000002,186.10000000000002,186.5,186.9,187.3,187.8,188.20000000000002,188.60000000000002,189.0,189.4,189.8,190.20000000000002,190.60000000000002,191.0,191.4,191.9,192.3,192.70000000000002,193.10000000000002,193.5,193.9,194.3,194.70000000000002,195.10000000000002,195.5,195.9,196.4,196.8,197.20000000000002,197.60000000000002,198.0,198.4,198.8,19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